CUED Publications database

Naturally occurring human urinary peptides for use in diagnosis of chronic kidney disease

Good, DM and Zürbig, P and Argilés, A and Bauer, HW and Behrens, G and Coon, JJ and Dakna, M and Decramer, S and Delles, C and Dominiczak, AF and Ehrich, JHH and Eitner, F and Fliser, D and Frommberger, M and Ganser, A and Girolami, MA and Golovko, I and Gwinner, W and Haubitz, M and Herget-Rosenthal, S and Jankowski, J and Jahn, H and Jerums, G and Julian, BA and Kellmann, M and Kliem, V and Kolch, W and Krolewski, AS and Luppi, M and Massy, Z and Melter, M and Neusüss, C and Novak, J and Peter, K and Rossing, K and Rupprecht, H and Schanstra, JP and Schiffer, E and Stolzenburg, JU and Tarnow, L and Theodorescu, D and Thongboonkerd, V and Vanholder, R and Weissinger, EM and Mischak, H and Schmitt-Kopplinr, P (2010) Naturally occurring human urinary peptides for use in diagnosis of chronic kidney disease. Molecular and Cellular Proteomics, 9. pp. 2424-2437. ISSN 1535-9476

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Abstract

Because of its availability, ease of collection, and correlation with physiology and pathology, urine is an attractive source for clinical proteomics/peptidomics. However, the lack of comparable data sets from large cohorts has greatly hindered the development of clinical proteomics. Here, we report the establishment of a reproducible, high resolution method for peptidome analysis of naturally occurring human urinary peptides and proteins, ranging from 800 to 17,000 Da, using samples from 3,600 individuals analyzed by capillary electrophoresis coupled to MS. All processed data were deposited in an Structured Query Language (SQL) database. This database currently contains 5,010 relevant unique urinary peptides that serve as a pool of potential classifiers for diagnosis and monitoring of various diseases. As an example, by using this source of information, we were able to define urinary peptide biomarkers for chronic kidney diseases, allowing diagnosis of these diseases with high accuracy. Application of the chronic kidney disease-specific biomarker set to an independent test cohort in the subsequent replication phase resulted in 85.5% sensitivity and 100% specificity. These results indicate the potential usefulness of capillary electrophoresis coupled to MS for clinical applications in the analysis of naturally occurring urinary peptides. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

Item Type: Article
Uncontrolled Keywords: Adult Aged Biomarkers Databases, Factual Electrophoresis, Capillary Female Humans Kidney Failure, Chronic Male Mass Spectrometry Middle Aged Peptides Proteomics ROC Curve Young Adult
Subjects: UNSPECIFIED
Divisions: UNSPECIFIED
Depositing User: Unnamed user with email sms67@cam.ac.uk
Date Deposited: 12 Mar 2019 01:08
Last Modified: 15 Apr 2021 02:52
DOI: 10.1074/mcp.M110.001917