CUED Publications database

Spatial heterogeneity of cell-matrix adhesive forces predicts human glioblastoma migration

Rezk, R and Jia, BZ and Wendler, A and Dimov, I and Watts, C and Markaki, AE and Franze, K and Kabla, AJ Spatial heterogeneity of cell-matrix adhesive forces predicts human glioblastoma migration. Neuro-Oncology Advances. (Unpublished)

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Background Glioblastoma (GBM) is a highly aggressive incurable brain tumor. The main cause of mortality in GBM patients is the invasive rim of cells migrating away from the main tumor mass and invading healthy parts of the brain. Although motion is driven by forces, our current understanding of the physical factors involved in glioma infiltration remains limited. This study aims to investigate the adhesion properties within and between patients’ tumors on a cellular level and test whether these properties correlate with cell migration. Methods Six tissue samples were taken from spatially separated sections during 5-aminolevulinic acid (5-ALA) fluorescence guided surgery. Navigated biopsy samples were collected from strongly fluorescent tumor cores, a weak fluorescent tumor rim, and non-fluorescent tumor margins. A microfluidics device was built to induce controlled shear forces to detach cells from monolayer cultures. Cells were cultured on low modulus polydimethylsiloxane representative of the stiffness of brain tissue. Cell migration and morphology were then obtained using time lapse microscopy. Results GBM cell populations from different tumor fractions of the same patient exhibited different migratory and adhesive behaviors. These differences were associated with sampling location and amount of 5-ALA fluorescence. Cells derived from weak- and non-fluorescent tumor tissue were smaller, adhered less well, and migrated quicker than cells derived from the strongly fluorescent tumor mass. Conclusion GBM tumors are biomechanically heterogeneous. Selecting multiple populations and broad location sampling are therefore important to consider for drug testing.

Item Type: Article
Divisions: Div C > Biomechanics
Div C > Materials Engineering
Depositing User: Cron Job
Date Deposited: 23 Jul 2020 08:08
Last Modified: 08 Apr 2021 05:45
DOI: 10.1093/noajnl/vdaa081