CUED Publications database

Anharmonic surface interactions for biomolecular screening and characterization.

Ghosh, SK and Ostanin, VP and Seshia, AA (2011) Anharmonic surface interactions for biomolecular screening and characterization. Anal Chem, 83. pp. 549-554.

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The acoustic response of conventional mechanical oscillators, such as a piezoelectric crystal, is predominantly harmonic at modest amplitudes. However, here, we observe from the electrical response that significant motional anharmonicity is introduced in the presence of attached analyte. Experiments were conducted with streptavidin-coated polystyrene microbeads of various sizes attached to a quartz crystal resonator via specific and nonspecific molecular tethers in liquid. Quantitative analysis reveals that the deviation of odd Fourier harmonics of the response caused by introduction of microbeads as a function of oscillation amplitude presents a unique signature of the molecular tether. Hence, the described anharmonic detection technique (ADT) based on this function allows screening of biomolecules and provides an additional level of selectivity in receptor-based detection that is often associated with nonspecific interactions. We also propose methods to extract mechanical force-extension characteristics of the molecular tether and activation energy using this technique.

Item Type: Article
Uncontrolled Keywords: Acoustics Microspheres Models, Molecular Physics Polystyrenes Quartz Shear Strength Streptavidin Surface Properties
Divisions: Div C > Applied Mechanics
Depositing User: Cron Job
Date Deposited: 09 Dec 2016 17:14
Last Modified: 16 Jan 2017 10:05