CUED Publications database

Probing protein aggregation with quartz crystal microbalances.

Knowles, TP and Devlin, GL and Dobson, CM and Welland, ME (2011) Probing protein aggregation with quartz crystal microbalances. Methods Mol Biol, 752. pp. 137-145.

Full text not available from this repository.

Abstract

The supra-molecular self-assembly of peptides and proteins is a process which underlies a range of normal and aberrant biological pathways in nature, but one which remains challenging to monitor in a quantitative way. We discuss the experimental details of an approach to this problem which involves the direct measurement in vitro of mass changes of the aggregates as new molecules attach to them. The required mass sensitivity can be achieved by the use of a quartz crystal transducer-based microbalance. The technique should be broadly applicable to the study of protein aggregation, as well as to the identification and characterisation of inhibitors and modulators of this process.

Item Type: Article
Uncontrolled Keywords: Animals Cattle Insulin Kinetics Protein Multimerization Protein Structure, Secondary Quartz Crystal Microbalance Techniques
Subjects: UNSPECIFIED
Divisions: Div B > Solid State Electronics and Nanoscale Science
Depositing User: Cron Job
Date Deposited: 07 Mar 2014 11:22
Last Modified: 17 Mar 2014 14:27
DOI: 10.1007/978-1-60327-223-0_9

Actions (login required)

View Item