Vanleene, M and Saldanha, Z and Cloyd, KL and Jell, G and Bou-Gharios, G and Bassett, JH and Williams, GR and Fisk, NM and Oyen, ML and Stevens, MM and Guillot, PV and Shefelbine, SJ (2011) Transplantation of human fetal blood stem cells in the osteogenesis imperfecta mouse leads to improvement in multiscale tissue properties. Blood, 117. pp. 1053-1060.Full text not available from this repository.
Osteogenesis imperfecta (OI or brittle bone disease) is a disorder of connective tissues caused by mutations in the collagen genes. We previously showed that intrauterine transplantation of human blood fetal stem/stromal cells in OI mice (oim) resulted in a significant reduction of bone fracture. This work examines the cellular mechanisms and mechanical bone modifications underlying these therapeutic effects, particularly examining the direct effects of donor collagen expression on bone material properties. In this study, we found an 84% reduction in femoral fractures in transplanted oim mice. Fetal blood stem/stromal cells engrafted in bones, differentiated into mature osteoblasts, expressed osteocalcin, and produced COL1a2 protein, which is absent in oim mice. The presence of normal collagen decreased hydroxyproline content in bones, altered the apatite crystal structure, increased the bone matrix stiffness, and reduced bone brittleness. In conclusion, expression of normal collagen from mature osteoblast of donor origin significantly decreased bone brittleness by improving the mechanical integrity of the bone at the molecular, tissue, and whole bone levels.
|Uncontrolled Keywords:||Animals Biomechanical Phenomena Blotting, Western Cell Differentiation Collagen Collagen Type I Cord Blood Stem Cell Transplantation Disease Models, Animal Female Femoral Fractures Femur Fetal Stem Cells Fractures, Bone Humans Male Mice Mice, Inbred C57BL Osteoblasts Osteocalcin Osteogenesis Imperfecta Pregnancy Principal Component Analysis Reverse Transcriptase Polymerase Chain Reaction Spectrum Analysis, Raman Transplantation, Heterologous|
|Depositing User:||Cron job|
|Date Deposited:||04 Feb 2015 22:07|
|Last Modified:||16 Feb 2015 01:26|