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A robust Bayesian two-sample test for detecting intervals of differential gene expression in microarray time series.

Stegle, O and Denby, KJ and Cooke, EJ and Wild, DL and Ghahramani, Z and Borgwardt, KM (2010) A robust Bayesian two-sample test for detecting intervals of differential gene expression in microarray time series. J Comput Biol, 17. pp. 355-367.

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Abstract

Understanding the regulatory mechanisms that are responsible for an organism's response to environmental change is an important issue in molecular biology. A first and important step towards this goal is to detect genes whose expression levels are affected by altered external conditions. A range of methods to test for differential gene expression, both in static as well as in time-course experiments, have been proposed. While these tests answer the question whether a gene is differentially expressed, they do not explicitly address the question when a gene is differentially expressed, although this information may provide insights into the course and causal structure of regulatory programs. In this article, we propose a two-sample test for identifying intervals of differential gene expression in microarray time series. Our approach is based on Gaussian process regression, can deal with arbitrary numbers of replicates, and is robust with respect to outliers. We apply our algorithm to study the response of Arabidopsis thaliana genes to an infection by a fungal pathogen using a microarray time series dataset covering 30,336 gene probes at 24 observed time points. In classification experiments, our test compares favorably with existing methods and provides additional insights into time-dependent differential expression.

Item Type: Article
Uncontrolled Keywords: Arabidopsis Area Under Curve Bayes Theorem Computational Biology Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Models, Genetic Multigene Family Normal Distribution Oligonucleotide Array Sequence Analysis Time Factors
Subjects: UNSPECIFIED
Divisions: Div F > Computational and Biological Learning
Depositing User: Cron Job
Date Deposited: 07 Mar 2014 11:44
Last Modified: 06 Oct 2014 01:22
DOI: 10.1089/cmb.2009.0175

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