CUED Publications database

Toxicity and imaging of multi-walled carbon nanotubes in human macrophage cells.

Cheng, C and Müller, KH and Koziol, KKK and Skepper, JN and Midgley, PA and Welland, ME and Porter, AE (2009) Toxicity and imaging of multi-walled carbon nanotubes in human macrophage cells. Biomaterials, 30. pp. 4152-4160.

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Multi-walled carbon nanotubes (MWNTs) have been proposed for use in many applications and concerns about their potential effect on human health have led to the interest in understanding the interactions between MWNTs and human cells. One important technique is the visualisation of the intracellular distribution of MWNTs. We exposed human macrophage cells to unpurified MWNTs and found that a decrease in cell viability was correlated with uptake of MWNTs due to mainly necrosis. Cells treated with purified MWNTs and the main contaminant Fe(2)O(3) itself yielded toxicity only from the nanotubes and not from the Fe(2)O(3). We used 3-D dark-field scanning transmission electron microscopy (DF-STEM) tomography of freeze-dried whole cells as well as confocal and scanning electron microscopy (SEM) to image the cellular uptake and distribution of unpurified MWNTs. We observed that unpurified MWNTs entered the cell both actively and passively frequently inserting through the plasma membrane into the cytoplasm and the nucleus. These suggest that MWNTs may cause incomplete phagocytosis or mechanically pierce through the plasma membrane and result in oxidative stress and cell death.

Item Type: Article
Uncontrolled Keywords: Animals Biocompatible Materials Cell Survival Cells, Cultured Electron Microscope Tomography Ferric Compounds Humans Macrophages Materials Testing Nanotubes, Carbon Necrosis Phagocytosis
Divisions: Div B > Solid State Electronics and Nanoscale Science
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:19
Last Modified: 31 May 2018 02:09