CUED Publications database

A microfluidic device for high density hydrodynamic cell trapping, growth and super-resolution imaging

Bell, LL and Seshia, AA and Laue, ED and Lando, D (2011) A microfluidic device for high density hydrodynamic cell trapping, growth and super-resolution imaging. Proceedings of IEEE Sensors. pp. 304-307.

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Abstract

Super-Resolution imaging techniques such as Fluorescent Photo-Activation Localisation Microscopy (FPALM) have created a powerful new toolkit for investigating living cells, however a simple platform for growing, trapping, holding and controlling the cells is needed before the approach can become truly widespread. We present a microfluidic device formed in polydimethylsiloxane (PDMS) with a fluidic design which traps cells in a high-density array of wells and holds them very still throughout the life cycle, using hydrodynamic forces only. The device meets or exceeds all the necessary criteria for FPALM imaging of Schizosaccharomyces pombe and is designed to remain flexible, robust and easy to use. © 2011 IEEE.

Item Type: Article
Subjects: UNSPECIFIED
Divisions: Div C > Applied Mechanics
Depositing User: Cron Job
Date Deposited: 07 Mar 2014 11:58
Last Modified: 29 Nov 2014 19:03
DOI: 10.1109/ICSENS.2011.6127209