Buell, AK and Dobson, CM and Welland, ME (2012) Measuring the kinetics of amyloid fibril elongation using quartz crystal microbalances. Methods Mol Biol, 849. pp. 101-119.Full text not available from this repository.
Kinetic measurements of amyloid growth provide insight into the free energy landscape of this supramolecular process and are crucial in the search for potent inhibitors of the main disorders with which it is associated, including Alzheimer's and Parkinson's diseases and Type II diabetes. In recent years, a new class of surface-bound biosensor assays, e.g., those based on surface plasmon resonance (SPR) and the quartz crystal microbalance (QCM) have been established as extremely valuable tools for kinetic measurements of amyloid formation. Here we describe detailed protocols of how QCM techniques can be used to monitor the elongation of amyloid fibrils in real time and to study the influence of external factors on the kinetics of amyloid growth with unprecedented accuracy.
|Uncontrolled Keywords:||Amyloid Amyloid beta-Peptides Animals Cattle Cystamine Enzyme Activation Gold Humans Hydrogen-Ion Concentration Insulin Kinetics Peptide Fragments Phosphatidylinositol 3-Kinases Protein Multimerization Protein Structure, Secondary Quartz Crystal Microbalance Techniques Surface Properties alpha-Synuclein src Homology Domains|
|Depositing User:||Cron job|
|Date Deposited:||16 Jul 2015 13:10|
|Last Modified:||31 Aug 2015 04:40|