CUED Publications database

Measuring the kinetics of amyloid fibril elongation using quartz crystal microbalances.

Buell, AK and Dobson, CM and Welland, ME (2012) Measuring the kinetics of amyloid fibril elongation using quartz crystal microbalances. Methods Mol Biol, 849. pp. 101-119.

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Abstract

Kinetic measurements of amyloid growth provide insight into the free energy landscape of this supramolecular process and are crucial in the search for potent inhibitors of the main disorders with which it is associated, including Alzheimer's and Parkinson's diseases and Type II diabetes. In recent years, a new class of surface-bound biosensor assays, e.g., those based on surface plasmon resonance (SPR) and the quartz crystal microbalance (QCM) have been established as extremely valuable tools for kinetic measurements of amyloid formation. Here we describe detailed protocols of how QCM techniques can be used to monitor the elongation of amyloid fibrils in real time and to study the influence of external factors on the kinetics of amyloid growth with unprecedented accuracy.

Item Type: Article
Uncontrolled Keywords: Amyloid Amyloid beta-Peptides Animals Cattle Cystamine Enzyme Activation Gold Humans Hydrogen-Ion Concentration Insulin Kinetics Peptide Fragments Phosphatidylinositol 3-Kinases Protein Multimerization Protein Structure, Secondary Quartz Crystal Microbalance Techniques Surface Properties alpha-Synuclein src Homology Domains
Subjects: UNSPECIFIED
Divisions: Div B > Solid State Electronics and Nanoscale Science
Depositing User: Cron Job
Date Deposited: 07 Mar 2014 11:27
Last Modified: 08 Dec 2014 02:27
DOI: 10.1007/978-1-61779-551-0_8