CUED Publications database

The influence of matrix integrity on stress-fiber remodeling in 3D.

Foolen, J and Deshpande, VS and Kanters, FMW and Baaijens, FPT (2012) The influence of matrix integrity on stress-fiber remodeling in 3D. Biomaterials, 33. pp. 7508-7518.

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Matrix anisotropy is important for long term in vivo functionality. However, it is not fully understood how to guide matrix anisotropy in vitro. Experiments suggest actin-mediated cell traction contributes. Although F-actin in 2D displays a stretch-avoidance response, 3D data are lacking. We questioned how cyclic stretch influences F-actin and collagen orientation in 3D. Small-scale cell-populated fibrous tissues were statically constrained and/or cyclically stretched with or without biochemical agents. A rectangular array of silicone posts attached to flexible membranes constrained a mixture of cells, collagen I and matrigel. F-actin orientation was quantified using fiber-tracking software, fitted using a bi-model distribution function. F-actin was biaxially distributed with static constraint. Surprisingly, uniaxial cyclic stretch, only induced a strong stretch-avoidance response (alignment perpendicular to stretching) at tissue surfaces and not in the core. Surface alignment was absent when a ROCK-inhibitor was added, but also when tissues were only statically constrained. Stretch-avoidance was also observed in the tissue core upon MMP1-induced matrix perturbation. Further, a strong stretch-avoidance response was obtained for F-actin and collagen, for immediate cyclic stretching, i.e. stretching before polymerization of the collagen. Results suggest that F-actin stress-fibers avoid cyclic stretch in 3D, unless collagen contact guidance dictates otherwise.

Item Type: Article
Uncontrolled Keywords: Collagen Extracellular Matrix Humans Imaging, Three-Dimensional Stress Fibers Stress, Mechanical
Divisions: Div C > Materials Engineering
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:56
Last Modified: 15 Sep 2020 04:39
DOI: 10.1016/j.biomaterials.2012.06.103