Martinez, VA and Besseling, R and Croze, OA and Tailleur, J and Reufer, M and Schwarz-Linek, J and Wilson, LG and Bees, MA and Poon, WCK (2012) Differential Dynamic Microscopy: a High-Throughput Method for Characterizing the Motility of Microorganism. Biophysical Journal, 103. pp. 1637-1647.Full text not available from this repository.
We present a fast, high-throughput method for characterizing the motility of microorganisms in 3D based on standard imaging microscopy. Instead of tracking individual cells, we analyse the spatio-temporal fluctuations of the intensity in the sample from time-lapse images and obtain the intermediate scattering function (ISF) of the system. We demonstrate our method on two different types of microorganisms: bacteria, both smooth swimming (run only) and wild type (run and tumble) Escherichia coli, and the bi-flagellate alga Chlamydomonas reinhardtii. We validate the methodology using computer simulations and particle tracking. From the ISF, we are able to extract (i) for E. coli: the swimming speed distribution, the fraction of motile cells and the diffusivity, and (ii) for C. reinhardtii: the swimming speed distribution, the amplitude and frequency of the oscillatory dynamics. In both cases, the motility parameters are averaged over \approx 10^4 cells and obtained in a few minutes.
|Divisions:||Div F > Signal Processing and Communications|
|Depositing User:||Cron Job|
|Date Deposited:||17 Jul 2013 10:10|
|Last Modified:||13 Jan 2014 01:21|
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