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A comprehensive study of lysozyme adsorption using dual polarization interferometry and quartz crystal microbalance with dissipation.

Xu, K and Ouberai, MM and Welland, ME (2013) A comprehensive study of lysozyme adsorption using dual polarization interferometry and quartz crystal microbalance with dissipation. Biomaterials, 34. pp. 1461-1470.

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Abstract

Protein adsorption plays a crucial role in biomaterial surface science as it is directly linked to the biocompatibility of artificial biomaterial devices. Here, elucidation of protein adsorption mechanism is effected using dual polarization interferometry and a quartz crystal microbalance to characterize lysozyme layer properties on a silica surface at different coverage values. Lysozyme is observed to adsorb from sparse monolayer to multilayer coverage. At low coverage an irreversibly adsorbed layer is formed with slight deformation consistent with side-on orientation. At higher coverage values dynamic re-orientation effects are observed which lead to monolayer surface coverages of 2-3 ng/mm² corresponding to edge-on or/and end-on orientations. These monolayer thickness values ranged between 3 and 4.5 nm with a protein density value of 0.60 g/mL and with 50 wt% solvent mass. Further increase of coverage results formation of a multilayer structure. Using the hydration content and other physical layer properties a tentative model lysozyme adsorption is proposed.

Item Type: Article
Uncontrolled Keywords: Adsorption Enzymes, Immobilized Equipment Design Equipment Failure Analysis Humans Interferometry Materials Testing Micro-Electrical-Mechanical Systems Muramidase Protein Binding Refractometry
Subjects: UNSPECIFIED
Divisions: Div B > Solid State Electronics and Nanoscale Science
Depositing User: Cron Job
Date Deposited: 07 Mar 2014 11:25
Last Modified: 25 Aug 2014 01:10
DOI: 10.1016/j.biomaterials.2012.10.078

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