CUED Publications database

A simple bioconjugate attachment protocol for use in single molecule force spectroscopy experiments based on mixed self-assembled monolayers.

Attwood, SJ and Simpson, AM and Stone, R and Hamaia, SW and Roy, D and Farndale, RW and Ouberai, M and E Welland, M (2012) A simple bioconjugate attachment protocol for use in single molecule force spectroscopy experiments based on mixed self-assembled monolayers. Int J Mol Sci, 13. pp. 13521-13541.

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Abstract

Single molecule force spectroscopy is a technique that can be used to probe the interaction force between individual biomolecular species. We focus our attention on the tip and sample coupling chemistry, which is crucial to these experiments. We utilised a novel approach of mixed self-assembled monolayers of alkanethiols in conjunction with a heterobifunctional crosslinker. The effectiveness of the protocol is demonstrated by probing the biotin-avidin interaction. We measured unbinding forces comparable to previously reported values measured at similar loading rates. Specificity tests also demonstrated a significant decrease in recognition after blocking with free avidin.

Item Type: Article
Uncontrolled Keywords: Avidin Biotin Gold Microscopy, Atomic Force Protein Binding Quartz Crystal Microbalance Techniques Surface Properties
Subjects: UNSPECIFIED
Divisions: UNSPECIFIED
Depositing User: Cron job
Date Deposited: 16 Jul 2015 13:32
Last Modified: 03 Aug 2015 06:37
DOI: 10.3390/ijms131013521