CUED Publications database

Immobilization of cell-binding peptides on poly-ε-caprolactone film surface to biomimic the peripheral nervous system.

de Luca, AC and Stevens, JS and Schroeder, SL and Guilbaud, JB and Saiani, A and Downes, S and Terenghi, G (2013) Immobilization of cell-binding peptides on poly-ε-caprolactone film surface to biomimic the peripheral nervous system. J Biomed Mater Res A, 101. pp. 491-501.

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Abstract

Cell-material interactions are crucial for cell adhesion and proliferation on biomaterial surfaces. Immobilization of biomolecules leads to the formation of biomimetic substrates, improving cell response. We introduced RGD (Arg-Gly-Asp) sequences on poly-ε-caprolactone (PCL) film surfaces using thiol chemistry to enhance Schwann cell (SC) response. XPS elemental analysis indicated an estimate of 2-3% peptide functionalization on the PCL surface, comparable with carbodiimide chemistry. Contact angle was not remarkably reduced; hence, cell response was only affected by chemical cues on the film surface. Adhesion and proliferation of Schwann cells were enhanced after PCL modification. Particularly, RGD immobilization increased cell attachment up to 40% after 6 h of culture. It was demonstrated that SC morphology changed from round to very elongated shape when surface modification was carried out, with an increase in the length of cellular processes up to 50% after 5 days of culture. Finally RGD immobilization triggered the formation of focal adhesion related to higher cell spreading. In summary, this study provides a method for immobilization of biomolecules on PCL films to be used in peripheral nerve repair, as demonstrated by the enhanced response of Schwann cells.

Item Type: Article
Uncontrolled Keywords: Animals Biocompatible Materials Cell Adhesion Cell Proliferation Cell Shape Focal Adhesions Immobilized Proteins Oligopeptides Peripheral Nervous System Photoelectron Spectroscopy Polyesters Rats Rats, Sprague-Dawley Schwann Cells Solvents Surface Properties Volatilization
Subjects: UNSPECIFIED
Divisions: Div C > Biomechanics
Depositing User: Cron Job
Date Deposited: 07 Mar 2014 11:23
Last Modified: 09 Jun 2014 01:02
DOI: 10.1002/jbm.a.34345

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