de Luca, AC and Terenghi, G and Downes, S (2014) Chemical surface modification of poly-ε-caprolactone improves Schwann cell proliferation for peripheral nerve repair. J Tissue Eng Regen Med, 8. pp. 153-163.Full text not available from this repository.
Poly-ε-caprolactone (PCL) is a biodegradable and biocompatible polymer used in tissue engineering for various clinical applications. Schwann cells (SCs) play an important role in nerve regeneration and repair. SCs attach and proliferate on PCL films but cellular responses are weak due to the hydrophobicity and neutrality of PCL. In this study, PCL films were hydrolysed and aminolysed to modify the surface with different functional groups and improve hydrophilicity. Hydrolysed films showed a significant increase in hydrophilicity while maintaining surface topography. A significant decrease in mechanical properties was also observed in the case of aminolysis. In vitro tests with Schwann cells (SCs) were performed to assess film biocompatibility. A short-time experiment showed improved cell attachment on modified films, in particular when amino groups were present on the material surface. Cell proliferation significantly increased when both treatments were performed, indicating that surface treatments are necessary for SC response. It was also demonstrated that cell morphology was influenced by physico-chemical surface properties. PCL can be used to make artificial conduits and chemical modification of the inner lumen improves biocompatibility.
|Uncontrolled Keywords:||Schwann cells biocompatibility nerve regeneration polycaprolactone surface modification wettability Amines Animals Cell Adhesion Cell Proliferation Cell Shape Hydrolysis Hydrophobic and Hydrophilic Interactions Nerve Regeneration Peripheral Nerves Polyesters Rats Rats, Sprague-Dawley Schwann Cells Spectroscopy, Fourier Transform Infrared Surface Properties Water|
|Divisions:||Div C > Biomechanics|
|Depositing User:||Cron Job|
|Date Deposited:||15 Dec 2015 12:54|
|Last Modified:||13 Feb 2016 05:18|