CUED Publications database

Electrostatic effects in filamentous protein aggregation.

Buell, AK and Hung, P and Salvatella, X and Welland, ME and Dobson, CM and Knowles, TP (2013) Electrostatic effects in filamentous protein aggregation. Biophys J, 104. pp. 1116-1126.

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Abstract

Electrostatic forces play a key role in mediating interactions between proteins. However, gaining quantitative insights into the complex effects of electrostatics on protein behavior has proved challenging, due to the wide palette of scenarios through which both cations and anions can interact with polypeptide molecules in a specific manner or can result in screening in solution. In this article, we have used a variety of biophysical methods to probe the steady-state kinetics of fibrillar protein self-assembly in a highly quantitative manner to detect how it is modulated by changes in solution ionic strength. Due to the exponential modulation of the reaction rate by electrostatic forces, this reaction represents an exquisitely sensitive probe of these effects in protein-protein interactions. Our approach, which involves a combination of experimental kinetic measurements and theoretical analysis, reveals a hierarchy of electrostatic effects that control protein aggregation. Furthermore, our results provide a highly sensitive method for the estimation of the magnitude of binding of a variety of ions to protein molecules.

Item Type: Article
Uncontrolled Keywords: Amyloid Animals Cattle Humans Insulin Kinetics Osmolar Concentration Polymerization Static Electricity
Subjects: UNSPECIFIED
Divisions: Div B > Solid State Electronics and Nanoscale Science
Depositing User: Cron Job
Date Deposited: 07 Mar 2014 11:29
Last Modified: 20 Oct 2014 01:07
DOI: 10.1016/j.bpj.2013.01.031