CUED Publications database

A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging

Bell, L and Seshia, A and Lando, D and Laue, E and Palayret, M and Lee, SF and Klenerman, D (2014) A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging. Sensors and Actuators, B: Chemical, 192. pp. 36-41. ISSN 0925-4005

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Abstract

We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of > 25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging. © 2013 The Authors. Published by Elsevier B.V. All rights reserved.

Item Type: Article
Subjects: UNSPECIFIED
Divisions: Div C > Applied Mechanics
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:13
Last Modified: 18 Nov 2017 22:02
DOI: