Lorber, B and Hsiao, WK and Hutchings, IM and Martin, KR (2014) Adult rat retinal ganglion cells and glia can be printed by piezoelectric inkjet printing. Biofabrication, 6. 015001-.Full text not available from this repository.
We have investigated whether inkjet printing technology can be extended to print cells of the adult rat central nervous system (CNS), retinal ganglion cells (RGC) and glia, and the effects on survival and growth of these cells in culture, which is an important step in the development of tissue grafts for regenerative medicine, and may aid in the cure of blindness. We observed that RGC and glia can be successfully printed using a piezoelectric printer. Whilst inkjet printing reduced the cell population due to sedimentation within the printing system, imaging of the printhead nozzle, which is the area where the cells experience the greatest shear stress and rate, confirmed that there was no evidence of destruction or even significant distortion of the cells during jet ejection and drop formation. Importantly, the viability of the cells was not affected by the printing process. When we cultured the same number of printed and non-printed RGC/glial cells, there was no significant difference in cell survival and RGC neurite outgrowth. In addition, use of a glial substrate significantly increased RGC neurite outgrowth, and this effect was retained when the cells had been printed. In conclusion, printing of RGC and glia using a piezoelectric printhead does not adversely affect viability and survival/growth of the cells in culture. Importantly, printed glial cells retain their growth-promoting properties when used as a substrate, opening new avenues for printed CNS grafts in regenerative medicine.
|Uncontrolled Keywords:||Animals Biomechanical Phenomena Bioprinting Cell Proliferation Cell Survival Male Neuroglia Rats Rats, Sprague-Dawley Retinal Ganglion Cells|
|Divisions:||Div E > Production Processes|
|Depositing User:||Cron job|
|Date Deposited:||04 Feb 2015 22:37|
|Last Modified:||29 May 2015 11:14|