CUED Publications database

Collagen type IV at the fetal-maternal interface.

Oefner, CM and Sharkey, A and Gardner, L and Critchley, H and Oyen, M and Moffett, A (2014) Collagen type IV at the fetal-maternal interface. Placenta, 36. pp. 59-68.

Full text not available from this repository.


INTRODUCTION: Extracellular matrix proteins play a crucial role in influencing the invasion of trophoblast cells. However the role of collagens and collagen type IV (col-IV) in particular at the implantation site is not clear. METHODS: Immunohistochemistry was used to determine the distribution of collagen types I, III, IV and VI in endometrium and decidua during the menstrual cycle and the first trimester of pregnancy. Expression of col-IV alpha chains during the reproductive cycle was determined by qPCR and protein localisation by immunohistochemistry. The structure of col-IV in placenta was examined using transmission electron microscopy. Finally, the expression of col-IV alpha chain NC1 domains and collagen receptors was localised by immunohistochemistry. RESULTS: Col-IV alpha chains were selectively up-regulated during the menstrual cycle and decidualisation. Primary extravillous trophoblast cells express collagen receptors and secrete col-IV in vitro and in vivo, resulting in the increased levels found in decidua basalis compared to decidua parietalis. A novel expression pattern of col-IV in the mesenchyme of placental villi, as a three-dimensional network, was found. NC1 domains of col-IV alpha chains are known to regulate tumour cell migration and the selective expression of these domains in decidua basalis compared to decidua parietalis was determined. DISCUSSION: Col-IV is expressed as novel forms in the placenta. These findings suggest that col-IV not only represents a structural protein providing tissue integrity but also influences the invasive behaviour of trophoblast cells at the implantation site.

Item Type: Article
Uncontrolled Keywords: Alpha(IV) NC1 domains Extracellular matrix Placental bed Reproductive cycle Trophoblast Chorionic Villi Collagen Type IV Decidua Embryo Implantation Female Humans Placenta Pregnancy Pregnancy Trimester, First Trophoblasts Up-Regulation
Divisions: Div C > Biomechanics
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:09
Last Modified: 21 Jun 2018 02:34