CUED Publications database

Investigating biomechanical noise in neuroblastoma cells using the quartz crystal microbalance.

Prasad, A and Huefner, A and Mahajan, S and Seshia, AA (2015) Investigating biomechanical noise in neuroblastoma cells using the quartz crystal microbalance. J R Soc Interface, 12.

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Quantifying cellular behaviour by motility and morphology changes is increasingly important in formulating an understanding of fundamental physiological phenomena and cellular mechanisms of disease. However, cells are complex biological units, which often respond to external environmental factors by manifesting subtle responses that may be difficult to interpret using conventional biophysical measurements. This paper describes the adaptation of the quartz crystal microbalance (QCM) to monitor neuroblastoma cells undergoing environmental stress wherein the frequency stability of the device can be correlated to changes in cellular state. By employing time domain analysis of the resulting frequency fluctuations, it is possible to study the variations in cellular motility and distinguish between different cell states induced by applied external heat stress. The changes in the frequency fluctuation data are correlated to phenotypical physical response recorded using optical microscopy under identical conditions of environmental stress. This technique, by probing the associated biomechanical noise, paves the way for its use in monitoring cell activity, and intrinsic motility and morphology changes, as well as the modulation resulting from the action of drugs, toxins and environmental stress.

Item Type: Article
Uncontrolled Keywords: biomechanical noise frequency stability neuroblastoma cells quartz crystal microbalance Biosensing Techniques Cell Line, Tumor Cell Movement Cell Size Elastic Modulus Equipment Design Equipment Failure Analysis Humans Micro-Electrical-Mechanical Systems Neuroblastoma Neurons Reproducibility of Results Sensitivity and Specificity Signal-To-Noise Ratio Stress, Mechanical
Divisions: Div C > Applied Mechanics
Div C > Materials Engineering
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:45
Last Modified: 19 Jul 2018 07:35