CUED Publications database

Emergence of homeostatic epithelial packing and stress dissipation through divisions oriented along the long cell axis

Wyatt, TPJ and Harris, AR and Lam, M and Cheng, Q and Bellis, J and Dimitracopoulos, A and Kabla, AJ and Charras, GT and Baum, B (2015) Emergence of homeostatic epithelial packing and stress dissipation through divisions oriented along the long cell axis. Proceedings of the National Academy of Sciences of the United States of America, 112. pp. 5726-5731. ISSN 0027-8424

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Cell division plays an important role in animal tissue morphogenesis, which depends, critically, on the orientation of divisions. In isolated adherent cells, the orientation of mitotic spindles is sensitive to interphase cell shape and the direction of extrinsic mechanical forces. In epithelia, the relative importance of these two factors is challenging to assess. To do this, we used suspended monolayers devoid of ECM, where divisions become oriented following a stretch, allowing the regulation and function of epithelial division orientation in stress relaxation to be characterized. Using this system, we found that divisions align better with the long, interphase cell axis than with the monolayer stress axis. Nevertheless, because the application of stretch induces a global realignment of interphase long axes along the direction of extension, this is sufficient to bias the orientation of divisions in the direction of stretch. Each division redistributes the mother cell mass along the axis of division. Thus, the global bias in division orientation enables cells to act collectively to redistribute mass along the axis of stretch, helping to return the monolayer to its resting state. Further, this behavior could be quantitatively reproduced using a model designed to assess the impact of autonomous changes in mitotic cell mechanics within a stretched monolayer. In summary, the propensity of cells to divide along their long axis preserves epithelial homeostasis by facilitating both stress relaxation and isotropic growth without the need for cells to read or transduce mechanical signals.

Item Type: Article
Uncontrolled Keywords: cell division mechanical feedback mitotic rounding morphogenesis quantitative biology Animals Cadherins Cell Division Cell Shape Dogs Epithelial Cells Epithelium Green Fluorescent Proteins Homeostasis Madin Darby Canine Kidney Cells Mitosis Morphogenesis Software Stress, Mechanical
Divisions: Div C > Biomechanics
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:07
Last Modified: 10 Apr 2021 23:05
DOI: 10.1073/pnas.1420585112