CUED Publications database

Gap geometry dictates epithelial closure efficiency

Ravasio, A and Cheddadi, I and Chen, T and Pereira, T and Ong, HT and Bertocchi, C and Brugues, A and Jacinto, A and Kabla, AJ and Toyama, Y and Trepat, X and Gov, N and Neves De Almeida, L and Ladoux, B (2015) Gap geometry dictates epithelial closure efficiency. Nature Communications, 6. 7683-.

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Closure of wounds and gaps in tissues is fundamental for the correct development and physiology of multicellular organisms and, when misregulated, may lead to inflammation and tumorigenesis. To re-establish tissue integrity, epithelial cells exhibit coordinated motion into the void by active crawling on the substrate and by constricting a supracellular actomyosin cable. Coexistence of these two mechanisms strongly depends on the environment. However, the nature of their coupling remains elusive because of the complexity of the overall process. Here we demonstrate that epithelial gap geometry in both in vitro and in vivo regulates these collective mechanisms. In addition, the mechanical coupling between actomyosin cable contraction and cell crawling acts as a large-scale regulator to control the dynamics of gap closure. Finally, our computational modelling clarifies the respective roles of the two mechanisms during this process, providing a robust and universal mechanism to explain how epithelial tissues restore their integrity.

Item Type: Article
Uncontrolled Keywords: Actomyosin Animals Cell Movement Computer Simulation Dogs Drosophila melanogaster Epithelial Cells Epithelium Fluorescent Antibody Technique In Vitro Techniques Intravital Microscopy Laser Therapy Madin Darby Canine Kidney Cells Microsurgery Wound Healing
Divisions: Div C > Biomechanics
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 18:57
Last Modified: 10 Apr 2021 23:05
DOI: 10.1038/ncomms8683