CUED Publications database

Assembly and positioning of actomyosin rings by contractility and planar cell polarity.

Sehring, IM and Recho, P and Denker, E and Kourakis, M and Mathiesen, B and Hannezo, E and Dong, B and Jiang, D (2015) Assembly and positioning of actomyosin rings by contractility and planar cell polarity. Elife, 4. e09206-.

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Abstract

The actomyosin cytoskeleton is a primary force-generating mechanism in morphogenesis, thus a robust spatial control of cytoskeletal positioning is essential. In this report, we demonstrate that actomyosin contractility and planar cell polarity (PCP) interact in post-mitotic Ciona notochord cells to self-assemble and reposition actomyosin rings, which play an essential role for cell elongation. Intriguingly, rings always form at the cells' anterior edge before migrating towards the center as contractility increases, reflecting a novel dynamical property of the cortex. Our drug and genetic manipulations uncover a tug-of-war between contractility, which localizes cortical flows toward the equator and PCP, which tries to reposition them. We develop a simple model of the physical forces underlying this tug-of-war, which quantitatively reproduces our results. We thus propose a quantitative framework for dissecting the relative contribution of contractility and PCP to the self-assembly and repositioning of cytoskeletal structures, which should be applicable to other morphogenetic events.

Item Type: Article
Uncontrolled Keywords: Ciona intestinalis PCP actomyosin ring biophysics cell biology contractility notochord self-organization/active gels structural biology Actomyosin Animals Biophysical Phenomena Cell Polarity Cell Shape Ciona intestinalis Muscle Contraction Notochord Protein Multimerization Protein Transport
Subjects: UNSPECIFIED
Divisions: Div C > Biomechanics
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 18:59
Last Modified: 23 Nov 2017 03:30
DOI: