CUED Publications database

An accelerated buoyancy adhesion assay combined with 3-D morphometric analysis for assessing osteoblast adhesion on microgrooved substrata.

Sobral, JM and Malheiro, VN and Clyne, TW and Harris, J and Rezk, R and O'Neill, W and Markaki, AE (2015) An accelerated buoyancy adhesion assay combined with 3-D morphometric analysis for assessing osteoblast adhesion on microgrooved substrata. J Mech Behav Biomed Mater, 60. pp. 22-37.

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Abstract

An accelerated negative buoyancy method has been developed to assess cell adhesion strength. This method has been used in conjunction with 3-D morphometric analysis to understand the effects of surface topology on cell response. Aligned micro-grooved surface topographies (with a range of groove depths) were produced on stainless steel 316L substrates by laser ablation. An investigation was carried out on the effect of the micro-grooved surface topography on cell adhesion strength, cell and nucleus volumes, cell phenotypic expression and attachment patterns. Increased hydrophobicity and anisotropic wettability was observed on surfaces with deeper grooves. A reduction was noted in cell volume, projected areas and adhesion sites for deeper grooves, linked to lower cell proliferation and differentiation rates and also to reduced adhesion strength. The results suggest that the centrifugation assay combined with three-dimensional cell morphometric analysis has considerable potential for obtaining improved understanding of the cell/substrate interface.

Item Type: Article
Uncontrolled Keywords: Cell adhesion Centrifugation Laser ablation Surface topography
Subjects: UNSPECIFIED
Divisions: Div E > Production Processes
Div C > Biomechanics
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:21
Last Modified: 23 Nov 2017 04:17
DOI: