CUED Publications database

Loss of functional Dicer in mouse radial glia cell-autonomously prolongs cortical neurogenesis.

Nowakowski, TJ and Mysiak, KS and O'Leary, T and Fotaki, V and Pratt, T and Price, DJ (2013) Loss of functional Dicer in mouse radial glia cell-autonomously prolongs cortical neurogenesis. Dev Biol, 382. pp. 530-537.

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Abstract

Radial glia of the mouse cerebral cortex emerge from neuroepithelial stem cells around embryonic day 11 and produce excitatory cortical neurons until a few days before birth. The molecular mechanisms that regulate the end of cortical neurogenesis remain largely unknown. Here we investigated if the Dicer-dependent microRNA (miRNA) pathway is involved. By electroporating a cre-recombinase expression vector into the cortex of E13.5 embryos carrying a conditional allele of Dicer1, we induced mosaic recombination causing Dicer1 deletion and reporter activation in a subset of radial glia. We analysed the long-term fates of their progeny. We found that mutant radial glia produced abnormally large numbers of Cux1-positive neurons, many of which populated the superficial cortical layers. Injections of the S-phase marker bromodeoxyuridine between postnatal days 3 and 14 showed that much of this population was generated postnatally. Our findings suggest a role for Dicer-dependent processes in limiting the timespan of cortical neurogenesis.

Item Type: Article
Uncontrolled Keywords: Dicer Mouse Neurogenesis Telencephalon microRNA Animals Cerebral Cortex DEAD-box RNA Helicases Embryo, Mammalian Mice MicroRNAs Neurogenesis Neuroglia Neurons Ribonuclease III Stem Cells
Subjects: UNSPECIFIED
Divisions: Div F > Control
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:54
Last Modified: 21 Sep 2017 01:36
DOI: