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Inhibition of rat recombinant GluN1/GluN2A and GluN1/GluN2B NMDA receptors by ethanol at concentrations based on the US/UK drink-drive limit

Otton, HJ and Janssen, A and O'Leary, T and Chen, PE and Wyllie, DJA (2009) Inhibition of rat recombinant GluN1/GluN2A and GluN1/GluN2B NMDA receptors by ethanol at concentrations based on the US/UK drink-drive limit. European Journal of Pharmacology, 614. pp. 14-21. ISSN 0014-2999

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Abstract

Many studies examine the actions of ethanol on N-methyl-D-aspartate (NMDA) receptors using concentrations that are highly toxic (≥ 100 mM). This study re-assesses the actions of ethanol at concentrations based around the US/UK 'drink-drive' limit (17 mM). Using two-electrode voltage-clamp recordings we examined the actions of ethanol on recombinant GluN1/GluN2A and GluN1/GluN2B NMDA receptors expressed in Xenopus laevis oocytes. We also investigated its actions on NMDA receptors containing GluN2A subunits with truncated or deleted carboxy terminal domains. Ethanol inhibition was voltage-independent and for GluN1/GluN2A NMDA receptors mean inhibition (20 mM at - 60 mV) was 9.5 ± 0.8% (n = 33) while corresponding values for GluN1/GluN2B NMDA receptors were 6.5 ± 0.8% (n = 21). EC50 values for glutamate at GluN1/GluN2A and glutamate and glycine at GluN1/GluN2B NMDA receptors were unaffected by the presence of ethanol. We did however observe a small increase in glycine potency, in the presence of ethanol, at GluN1/GluN2A NMDA receptors. Neither voltage-dependent Mg2+ block nor memantine block was affected by ethanol. Reduced ethanol inhibition was observed however at NMDA receptors containing GluN2A subunits with mutated carboxy terminal domains. We conclude that the levels of inhibition seen with ethanol concentrations near to the US/UK drink-driving limit are very modest and even at higher (intoxicating) concentrations do not alter characteristic NMDA receptor properties. © 2009 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Alcohol Drinking Animals Automobile Driving Dose-Response Relationship, Drug Electric Conductivity Ethanol Hippocampus Neurons Protein Structure, Tertiary Rats Receptors, N-Methyl-D-Aspartate Recombinant Proteins United Kingdom United States
Subjects: UNSPECIFIED
Divisions: Div F > Control
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:54
Last Modified: 24 Oct 2019 13:29
DOI: 10.1016/j.ejphar.2009.04.034