CUED Publications database

Detection of atomic scale changes in the free volume void size of three-dimensional colorectal cancer cell culture using positron annihilation lifetime spectroscopy.

Axpe, E and Lopez-Euba, T and Castellanos-Rubio, A and Merida, D and Garcia, JA and Plaza-Izurieta, L and Fernandez-Jimenez, N and Plazaola, F and Bilbao, JR (2013) Detection of atomic scale changes in the free volume void size of three-dimensional colorectal cancer cell culture using positron annihilation lifetime spectroscopy. PLoS One, 9. e83838-.

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Abstract

Positron annihilation lifetime spectroscopy (PALS) provides a direct measurement of the free volume void sizes in polymers and biological systems. This free volume is critical in explaining and understanding physical and mechanical properties of polymers. Moreover, PALS has been recently proposed as a potential tool in detecting cancer at early stages, probing the differences in the subnanometer scale free volume voids between cancerous/healthy skin samples of the same patient. Despite several investigations on free volume in complex cancerous tissues, no positron annihilation studies of living cancer cell cultures have been reported. We demonstrate that PALS can be applied to the study in human living 3D cell cultures. The technique is also capable to detect atomic scale changes in the size of the free volume voids due to the biological responses to TGF-β. PALS may be developed to characterize the effect of different culture conditions in the free volume voids of cells grown in vitro.

Item Type: Article
Uncontrolled Keywords: Cell Culture Techniques Cell Line, Tumor Colorectal Neoplasms Humans Microscopy, Fluorescence Spectrum Analysis Spheroids, Cellular Tumor Cells, Cultured
Subjects: UNSPECIFIED
Divisions: Div C > Biomechanics
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:16
Last Modified: 14 Nov 2017 02:20
DOI: