CUED Publications database

Dipicolinic acid as a novel spore-inspired excipient for antibody formulation

Batalha, IL and Ke, P and Tejeda-Montes, E and Uddin, S and van der Walle, CF and Christie, G (2017) Dipicolinic acid as a novel spore-inspired excipient for antibody formulation. International Journal of Pharmaceutics, 526. pp. 332-338.

Full text not available from this repository.


Ionic excipients are commonly used in aqueous therapeutic monoclonal antibody (mAb) formulations. Novel excipients are of industrial interest, with a recent focus on Arg salt forms and their application as viscosity reducing and stabilizing additives. Here, we report that the calcium salt of dipicolinic acid (DPA, pyridine-2,6-dicarboxylic acid), uniquely present in nature in the core of certain bacterial spores, reduces the viscosity of a mAb formulated at 150mg/mL, below that achieved by Arg hydrochloride at the same concentration (10mM). DPA also reduced the reversible phase separation of the same formulation, which characteristically occurs for this mAb upon cooling to 4°C. Differential scanning calorimetry and differential scanning fluorimetry did not reveal a conformation destabilisation of the mAb in the presence of 10mM DPA, or by the related quinolinic acid (QA, pyridine-2,3-dicarboxylic acid). However, fluorescence spectrophotometry did reveal localised (aromatic) conformational changes to the mAb attributed to DPA, dependent on the salt form. While precise mechanisms of action remain to be identified, our preliminary data suggest that these DPA salts are worthy of further investigation as novel ionic excipient for biologics formulation.

Item Type: Article
Uncontrolled Keywords: antibody formulation bacterial spores dipicolinic acid novel excipients
Divisions: Div B > Solid State Electronics and Nanoscale Science
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 18:57
Last Modified: 15 Apr 2021 05:08
DOI: 10.1016/j.ijpharm.2017.05.012