CUED Publications database

In vitro studies with mammalian cell lines and gum arabic-coated magnetic nanoparticles

Bicho, A and Roque, ACA and Cardoso, AS and Domingos, P and Batalha, IL (2010) In vitro studies with mammalian cell lines and gum arabic-coated magnetic nanoparticles. Journal of Molecular Recognition, 23. pp. 536-542. ISSN 0952-3499

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Iron oxide magnetic nanoparticles (MNPs) were synthesized by the chemical co-precipitation method and coated with gum arabic (GA) by physical adsorption and covalent attachment. Cultures of mammalian cell lines (HEK293, CHO and TE671) were grown in the presence of uncoated and GA-coated MNPs. Cellular growth was followed by optical microscopy in order to assess the proportion of cells with particles, alterations in cellular density and the presence of debris. The in vitro assays demonstrated that cells from different origins are affected differently by the presence of the nanoparticles. Also, the methods followed for GA coating of MNPs endow distinct surface characteristics that probably underlie the observed differences when in contact with the cells. In general, the nanoparticles to which the GA was adsorbed had a smaller ability to attach to the cells' surface and to compromise the viability of the cultures. © 2010 John Wiley & Sons, Ltd.

Item Type: Article
Uncontrolled Keywords: Animals CHO Cells Cell Count Cell Culture Techniques Cell Line Cell Proliferation Coated Materials, Biocompatible Cricetinae Cricetulus Eukaryotic Cells Gum Arabic Humans Magnetics Magnetite Nanoparticles Mammals Time Factors
Divisions: Div B > Solid State Electronics and Nanoscale Science
Depositing User: Cron Job
Date Deposited: 17 Jul 2017 19:13
Last Modified: 10 Apr 2021 00:46
DOI: 10.1002/jmr.1066