CUED Publications database

Extracellular vesicles are independent metabolic units with asparaginase activity

Iraci, N and Gaude, E and Leonardi, T and Costa, ASH and Cossetti, C and Peruzzotti-Jametti, L and Bernstock, JD and Saini, HK and Gelati, M and Vescovi, AL and Bastos, C and Faria, N and Occhipinti, LG and Enright, AJ and Frezza, C and Pluchino, S (2017) Extracellular vesicles are independent metabolic units with asparaginase activity. Nature Chemical Biology, 13. pp. 951-955.

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Extracellular vesicles (EVs) are membrane particles involved in the exchange of a broad range of bioactive molecules between cells and the microenvironment. Although it has been shown that cells can traffic metabolic enzymes via EVs, much remains to be elucidated with regard to their intrinsic metabolic activity. Accordingly, herein we assessed the ability of neural stem/progenitor cell (NSC)-derived EVs to consume and produce metabolites. Our metabolomics and functional analyses both revealed that EVs harbor L-asparaginase activity, catalyzed by the enzyme asparaginase-like protein 1 (Asrgl1). Critically, we show that Asrgl1 activity is selective for asparagine and is devoid of glutaminase activity. We found that mouse and human NSC EVs traffic Asrgl1. Our results demonstrate, for the first time, that NSC EVs function as independent metabolic units that are able to modify the concentrations of critical nutrients, with the potential to affect the physiology of their microenvironment.

Item Type: Article
Uncontrolled Keywords: Asparaginase Extracellular Vesicles Models, Biological
Depositing User: Unnamed user with email
Date Deposited: 25 Jul 2017 03:53
Last Modified: 09 Sep 2021 02:16
DOI: 10.1038/nchembio.2422