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Interfacing Polymers and Tissues: Quantitative Local Assessment of the Foreign Body Reaction of Mononuclear Phagocytes to Polymeric Materials

Giusto, E and Donegà, M and Dumitru, AC and Foschi, G and Casalini, S and Bianchi, M and Leonardi, T and Russo, A and Occhipinti, LG and Biscarini, F and Garcia, R and Pluchino, S (2017) Interfacing Polymers and Tissues: Quantitative Local Assessment of the Foreign Body Reaction of Mononuclear Phagocytes to Polymeric Materials. Advanced Biosystems, 1. p. 1700021. ISSN 2366-7478

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Abstract

A quantitative method to assess the in vitro foreign body reaction (FBR) of mononuclear phagocytes (MP) to polymers relevant in implants for prosthetics, advanced therapies, and regenerative medicine is presented. It integrates single‐cell force spectroscopy (SCFS) with immunogenic profiles of the MPs. In cell force spectroscopy experiments a single phagocyte, linked at the end of an atomic force microscopy cantilever, probes the adhesion forces between the cell and the polymer surface. SCFS measures adhesion forces in a range from 10 pN to 100 nN and with spatial resolution from cell size down to nanometers, accessing the early adhesion events established at contact times between milliseconds and minutes. The time evolution within the first 60 s of the adhesion force between the phagocyte and the polymer surface before and after the treatment with an immunosuppressive drug, viz. Minocycline, a Federal Drug Administration (FDA)‐approved third generation tetracycline with anti‐inflammatory effects, is then studied. The adhesion force values measured at the single cell level is shown to correlate to the immunogenic profiles obtained by analysis of biomarkers and morphology of the MPs in culture. Also, Minocycline causes a decrease of both proinflammatory gene expression profiles and adhesive forces of single cells

Item Type: Article
Subjects: UNSPECIFIED
Divisions: UNSPECIFIED
Depositing User: Cron Job
Date Deposited: 12 Nov 2018 20:11
Last Modified: 18 Feb 2021 17:59
DOI: 10.1002/adbi.201700021